Assessment of stem cell and brain stroke
Assessment of marrow derived stem cell implantation in experimentally induced brain stroke and retinal ischemia mouse model
We have established the experimental model of neuronal damage in mice by middle cerebral artery (MCA) occlusion method and assessment of neuronal and ganglion cell damage by infarct area and neurobehavioral measurement, and investigated the effects of marrow derived mononuclear cell (MNC) and retinal stem cells by transplantation strategies. The adult Swiss albino male mice were used in which the right common carotid artery, external carotid artery and internal carotid artery (ICA) were exposed via a midline incision.
An 8-0 polypropylene suture coated with poly-lysine was inserted into ICA and advanced into the origin of MCA. The animals were subjected to MCA occlusion for 60 minutes followed by 23 h reperfusion. After 24 h, coronal sections as well as retinal sections were obtained and stained with 1{b1db15a8718e72bc8a492e4522ac741b8363f8278d1cb30882962c0bf10cf6a3} triphenyltetrazolium chloride (TTC), a marker of cerebral ischemia damage in mice brain.
The marrow derived mononuclear cells were injected into ischemic injured mice through tail vein route. The experimental mice showed a contra lateral turning behavior which persisted up to 4 h after MCAO which was considered as a precocious index of neurological deficit and neuronal damage. The ganglion layer depletion results due to retinal ischemia. The retinal stem cells were implanted and followed temporally in order to understand the efficacy of these cells in rescuing the function deficit caused due to ischemia. This is an excellent platform for analysis of the effects of stem cells and drugs on the ischemic injury.
